• Product Namebeta IV Tubulin antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 238 and 420 of Human TUBB4
• Target Namebeta IV Tubulin
• Alternative Names    TUBB5; beta-5
• Accession No. Swiss-Prot:P04350
  Gene ID:10382
• UniprotP04350
• Gene ID10382;
• Concentration 0.4mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 50kd
  Western blotting: 1:500-1:3000
  Immunofluorescence: 1:100-1:200
  

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

• Sample(30 ug of whole cell lysate)
  A: 293T
  7.5% SDS PAGE
  Primary antibody diluted at 1: 1000
• Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using beta Tubulin 4 antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 2000.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting