• Product NameCMAS antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 69 and 384 of CMAS
• Target NameCMAS
• Accession No. NCBI Gene ID: 55907
  NCBI mRNA#: NM_018686
  NCBI Protein#: NP_061156
• UniprotQ8NFW8
• Gene ID55907;
• Concentration 1mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 48kd
  Western blotting: 1:500-1:3000
  Immunofluorescence: 1:100-1:200
  

The enzyme encoded by this gene catalyzes the activation of Neu5Ac to Cytidine 5-prime-monophosphate N-acetylneuraminic acid (CMP-Neu5Ac), which provides the substrate required for the addition of sialic acid. Sialic acids of cell surface glycoproteins and glycolipids play a pivotal role in the structure and function of animal tissues. The pattern of cell surface sialylation is highly regulated during embryonic development, and changes with stages of differentiation. Studies of a similar murine protein suggest that this protein localizes to the nucleus. [provided by RefSeq]

• Sample(30 ug of whole cell lysate)
  A: 293T
  B: HeLa S3
  7.5% SDS PAGE
  Primary antibody diluted at 1: 1500
• Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using CMAS antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 2000.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting