• Product NameSec61 alpha-1 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC IF
• Species ReactivityHu
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide contain a sequence corresponding to a region within amino acids 413 and 476 of Sec61 alpha-1
• Target NameSec61 alpha-1
• Accession No. Swiss-Prot:P61619
  Gene ID:29927
• UniprotP61619
• Gene ID29927;
• Concentration 1mg/ml
• Formulation Supplied in 1XPBS, 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 52kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:50-1:500
  Immunofluorescence: 1:100-1:200
  

The protein encoded by this gene belongs to the SECY/SEC61- alpha family. It appears to play a crucial role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. This protein found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins. This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: K562
  10% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SEC61A1 antibody at 1: 500 dilution.
• Immunofluorescence analysis of methanol-fixed A431, using SEC61A1 antibody at 1: 200 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting