• Product Namea-catenin(Phospho-Ser641) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of a-catenin only when phosphorylated at serine 641.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 641 (D-D-S(p)-D-F) derived from Human a-catenin.
• Target Namea-catenin
• ModificationPhospho
• Alternative Names    Cadherin-associated protein; Alpha E-catenin; NY-REN-13 antigen
• Accession No. Swiss-Prot: P35221
  NCBI Protein: NP_001894.2
• UniprotP35221
• Gene ID1495;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 100kd
  Western blotting: 1:500~1:1000

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. May play a crucial role in cell differentiation.

Hwang, S.G. et al. (2005) J. Biol. Chem. 280, 12758-12765
Drees, F. et al. (2005) Cell 123, 903-915.
Yamada, S. et al. (2005) Cell 123, 889-901.
Kobielak, A. and Fuchs, E. (2004) Nat. Rev. Mol. Cell Biol. 5, 614-625.

• Western blot analysis of extracts from Hela cells untreated or treated with starvation using a-catenin(Phospho-Ser641) antibody #11330.
• Western blot analysis of extracts from HT29 cells, treated with serum or calf intestinal phosphatase (CIP), using α-catenin (Phospho-Ser641) Antibody #11330.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting