• Product NameTwist1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total Twist1 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.18~22(S-N-S-E-E) derived from Human Twist1
• Target NameTwist1
• Alternative Names    SCS; ACS3; CRS1; BPES2; BPES3
• Accession No. Swiss-Prot: Q15672
  NCBI Protein: NP_000465.1
• UniprotQ15672
• Gene ID7291;
• Calculated MW 28KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Western blotting      1:500-1:1000
  Immunohistochemistry  1:100-1:500

Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.

"Twist regulates cytokine gene expression through a negative feedback loop that represses NF-kappaB activity."Sosic D., Richardson J.A., Yu K., Ornitz D.M., Olson E.N.Cell 112:169-180(2003) 
"Mutations of the TWIST gene in the Saethre-Chotzen syndrome."el Ghouzzi V., le Merrer M., Perrin-Schmitt F., et al., Nat. Genet. 15:42-46(1997)

• Western blot analysis of extracts from JK, Raji and HL-60 cells using Twist1 Antibody #21642.
• Twist1 Antibody staining Twist in Mouse pancreatic cancer tissue sections by Immunohistochemistry

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting