• Product NameIGF-1R(Ab-1161) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total IGF-1R protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.1159~1163 (D-I-Y-E-T) derived from Human IGF-1R .
• Target NameIGF-1R
• Alternative Names    Insulin-like growth factor I receptor; CD221; IGF1R; kinase IGF1R;
• Accession No. Swiss-Prot: P08069
  NCBI Protein: NP_000866.1
• UniprotP08069
• Gene ID3480;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 200 95 kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1.

Li S, et al. (1994) J Biol Chem; 269(51).
Hernandez-Sanchez C, et al. (1995) J Biol Chem.

• Western blot analysis of extracts from 293 cells using IGF-1R(Ab-1161) Antibody #21080 and the same antibody preincubated with blocking peptide.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IGF-1R(Ab-1161) Antibody #21080(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed MCF cells using IGF-1R(Ab-1161) Antibody #21080.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting