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PGC-1 alpha Monoclonal Antibody

27135
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27135 100ul $298.00

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Description
  • Product Name PGC-1 alpha Monoclonal Antibody
  • Host Species Mouse
  • Clonality Monoclonal
  • Purification Affinity-chromatography
  • Applications ELISA WB
  • Species Reactivity Hu
  • Specificity PGC-1alpha antibody detects endogenous levels of total PGC-1alpha
  • Immunogen Type Recombinant Protein
  • Immunogen Description Purified recombinant fragment of human PGC-1alpha expressed in E. Coli
  • Target Name PGC-1 alpha
  • Alternative Names PPARGC1A; LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1(alpha)
  • Accession No. Swiss-Prot#: Q9UBK2
  • SDS-PAGE MW 91KD
  • Concentration 1.0mg/ml
  • Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt
  • Storage Store at -20°C/1 year
Application Details

 ELISA: 1:10000 
 WB: 1:500 - 1:2000

Images
  • PGC-1 alpha Monoclonal Antibody  - SAB | Signalway Antibody

    Western blot analysis using PGC-1alpha mAb against human PGC-1alpha (AA: 570-798) recombinant protein. (Expected MW is 70 kDa)

Background

The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPAR gamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. 

    Please let us know if you have published research using #27135 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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