• Product NameFKHR(Ab-319) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total FKHR protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 317~321 (T-S-S-N-A) derived from Human FKHR.
• Target NameFKHR
• Alternative Names    FKHR; FOXO1;
• Accession No. Swiss-Prot: Q12778
  NCBI Protein: NP_002006.2
• UniprotQ12778
• Gene ID2308;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 78-82 kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

Transcription factor.

• Western blot analysis of extracts from 3T3 and PC12 cells using FKHR(Ab-319) Antibody #21161.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHR(Ab-319) Antibody #21161(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting