• Product NameStathmin1(Ab-25) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total Stathmin 1 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 23~27 (I-L-S-P-R) derived from Human Stathmin 1.
• Target NameStathmin1
• Alternative Names    STMN1; STN1; stathmin
• Accession No. Swiss-Prot: P16949
  NCBI Protein: NP_001138926.1
• UniprotP16949
• Gene ID3925;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 19kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear

Boehm M, et al. (2002) EMBO J 21(13): 3390-3401.
Vadlamudi RK, et al. (2005) Mol Cell Biol 25(9): 3726-3736.
Zilfou JT, et al. (2001) Mol Cell Biol 21(12): 3974-3985.
Biernat J, et al. (2002) Mol Biol Cell 13(11): 4013-4028.

• Western blot analysis of extracts from 293 and Hela cells using Stathmin 1(Ab-25) Antibody #21217.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Stathmin 1(Ab-25) Antibody #21217(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using Stathmin 1(Ab-25) Antibody #21217.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting