• Product NameGAP43(Ab-41) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total GAP43 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.38~43 (Q-A-S-F-R) derived from Human GAP43.
• Target NameGAP43
• Alternative Names    B-50; BASP2; NEUM; PP46; axonal membrane protein GAP-43
• Accession No. Swiss-Prot: P17677
  NCBI Protein: NP_001123536.1
• UniprotP17677
• Gene ID2596;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 43kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

GAP43 encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Rachael L. Neve, et,al. (1998) J. Neurosci; 18: 7757.
Yiping Shen, et,al. (2002) J. Neurosci; 22: 239.
Chantal Gamby, et,al. (1996) J. Biol. Chem; 271: 26698.

• Western blot analysis of extracts from mouse brain tissue using GAP43(Ab-41) Antibody #21273 and the same antibody preincubated with blocking peptide.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GAP43(Ab-41) Antibody #21273(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using GAP43(Ab-41) Antibody #21273.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting