• Product NameMelanoma-associated antigen D1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total Melanoma-associated antigen D1 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide with sequence C-EAEARAEARNRMGIGDE derived from Mouse Melanoma-associated antigen D1.
• Target NameMelanoma-associated antigen D1
• Alternative Names    MAGED1
• Accession No. Swiss-Prot: Q9QYH6
  NCBI Protein: NP_062765.1
• UniprotQ9QYH6
• Gene ID94275;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 95kd
  Western blotting: 1:500~1:1000

Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Binds p75NTR and antagonizes its association with TrkA, inhibits cell cycle progression, and facilitates p75NTR-mediated apoptosis. May act as a regulator of the function of DLX family members. May regulate p53 transcriptional activity and inhibit cell proliferation. Enhances p53 phosphorylation and accumulation.

Zhao Z,et al.(2002) Sci China C Life Sci.45(4):412-20.
Shen WG,et al.(2007) Cell Commun Adhes.14(1):21-31.
Cheresh DA,et al. (1984) J Biol Chem.259(12):7453-9.

• Western blot analysis of extracts from PC12 cells using Melanoma-associated antigen D1 Antibody #21408 and the same antibody preincubated with blocking peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting