• Product NameH2B Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total H2B protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.41~45 (Y-V-Y-K-V) derived from Human H2B.
• Target NameH2B
• Alternative Names    H2BQ; GL105; H2B.1; H2BFQ; H2BGL105
• Accession No. Swiss-Prot: P33778
  NCBI Protein: NP_003519.1
• UniprotP33778
• Gene ID3018;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 14kd
  Western blotting: 1:500~1:1000

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Borchert GM,et al. (2010) PLoS One.5(7):e11641.
Pennini ME,et al.(2010) PLoS Pathog.6(7):e1000995.
Lu C,et al. (2010) Sci China Life Sci.53(6):663-8.

• Western blot analysis of extracts from Hela and 293 cells using H2B antibody #21417.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting