• Product NameFibrillarin Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total Fibrillarin protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.297~301( L-T-L-E-P) derived from Human Fibrillarin
• Target NameFibrillarin
• Alternative Names    FBL; FIB; FLRN; RNU3IP1;
• Accession No. Swiss-Prot: P22087
  NCBI Protein: NP_001427.2
• UniprotP22087
• Gene ID2091;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 37kd
  Western blotting: 1:500~1:1000
  

Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.

Lischwe M.A., Ochs R.L., Reddy R., Cook R.G., Yeoman L.C., Tan E.M., Reichlin M., Busch H. J. Biol. Chem. 260:14304-14310(1985)
Yanagida M., Hayano T., Yamauchi Y., Shinkawa T., Natsume T., Isobe T., Takahashi N.J. Biol. Chem. 279:1607-1614(2004)
Wang Y., Liu J., Zhao H., Lue W., Zhao J., Yang L., Li N., Du X., Ke Y.Biochim. Biophys. Acta 1773:863-868(2007)

• Western blot analysis of extract from 293, Hela cells using Fibrillarin Antibody #21495

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting