• Product NameELOA1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total ELOA1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human ELOA1.
• Target NameELOA1
• Alternative Names    Transcription elongation factor B polypeptide 3; RNA polymerase II transcription factor SIII subunit A1; SIII p110; Elongin-A; EloA
• Accession No. Swiss-Prot: Q14241
  NCBI Gene ID: 6924
• UniprotQ14241
• Gene ID6924;
• SDS-PAGE MW 87kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).

Aso T., Gene 168:277-278(1996).
Gregory S.G., Nature 441:315-321(2006).
Olsen J.V., Cell 127:635-648(2006)

• Western blot analysis of extracts from Jurkat cells and HUVEC cells, using ELOA1 antibody #34267.
• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ELOA1 antibody #34267.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting