• Product Namea-Tubulin Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total alpha-Tubulin protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.437-441(V-D-S-V-E) derived from Human alpha-Tubulin.
• Target Namea-Tubulin
• Alternative Names    K-ALPHA-1
• Accession No. Swiss-Prot: P68363
  NCBI Protein: NP_006073.2
• UniprotP68363
• Gene ID10376;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 52kd
  Western blotting: 1:500~1:1000
  

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the a-chain.

Cowan N.J., Dobner P., Fuchs E.V., Cleveland D.W.Mol. Cell. Biol. 3:1738-1745(1983)

• Western blot analysis of extracts from Hela and HT29 cells using alpha-Tubulin Antibody #21581.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting