• Product NameALY antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 203 of ALY
• Target NameALY
• Accession No. Swiss-Prot:Q86V81
  Gene ID:10189
• UniprotQ86V81
• Gene ID10189;
• Concentration 0.6mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 27kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:100-1:500
  Immunofluorescence: 1:100-1:200
  

The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: Molt-4
  12% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunohistochemical analysis of paraffin-embedded SAS xenograft, using ALY antibody at 1: 500 dilution.
• Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using ALY antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 2000.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting