• Product NameAPI5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total API5 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human apoptosis inhibitor 5
• Target NameAPI5
• Alternative Names    AAC11; AAC-11
• Accession No. Swiss-Prot#: Q9BZZ5
  NCBI Gene ID: 8539
  Gene Accssion: NP_006586
• UniprotQ9BZZ5
• Gene ID8539;
• SDS-PAGE MW 57kd
• Concentration 2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  

This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates Acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

• Gel: 10%SDS-PAGE
  Lysates (from left to right): 293T and Hela cell
  Amount of lysate: 30ug per lane
  Primary antibody: 1/250 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 2 minutes

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting