• Product NamePXN Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total PXN protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the N terminal of human Paxillin
• Target NamePXN
• Alternative Names    FLJ16691; PAXI; Paxillin; PXN
• Accession No. Swiss-Prot#: F5GZ78
  NCBI Gene ID: 5829
  Gene Accssion: NP_002850
• UniprotP49023
• Gene ID5829;
• SDS-PAGE MW 66kd
• Concentration 1.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445).

• Gel: 10%SDS-PAGE
  Lysate: 40ug Hela cell
  Primary antibody: 1/700 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 3 minutes
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #37012 at dilution 1/40.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting