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LIN28B Antibody

37362
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37362 100ul $279.00

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Description
  • Product Name LIN28B Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antigen affinity purification.
  • Applications WB
  • Species Reactivity Hu Ms
  • Specificity The antibody detects endogenous levels of total LIN28B protein.
  • Immunogen Type Peptide
  • Immunogen Description Synthetic peptide of human LIN28B
  • Target Name LIN28B
  • Alternative Names CSDD2
  • Accession No. Swiss-Prot#: Q6ZN17
    NCBI Gene ID: 389421
    Gene Accssion: NP_001004317
  • SDS-PAGE MW 27kd
  • Concentration 1.6mg/ml
  • Formulation Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:500-1:2000

Images
  • LIN28B  Antibody - SAB | Signalway Antibody

    Gel: 10%SDS-PAGE
    Lysates (from left to right): Mouse liver tissue and k562 cell, hela and 293T cell
    Amount of lysate: 40ug per lane
    Primary antibody: 1/550 dilution
    Secondary antibody dilution: 1/8000
    Exposure time: 1 minute

Background

The protein encoded by this gene belongs to the lin-28 family, which is characterized by the presence of a cold-shock domain and a pair of CCHC zinc finger domains. This gene is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines. It is negatively regulated by microRNAs that target sites in the 3' UTR, and overexpression of this gene in primary tumors is linked to the repression of let-7 family of microRNAs and derepression of let-7 targets, which facilitates cellular transformation. [provided by RefSeq, Jun 2012]

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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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