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KDM5B Antibody

37681
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  Catalog No. Package Size Price
37681 100ul $279.00

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Description
  • Product Name KDM5B Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antigen affinity purification.
  • Applications WB IHC
  • Species Reactivity Hu Ms
  • Specificity The antibody detects endogenous levels of total KDM5B protein.
  • Immunogen Type Peptide
  • Immunogen Description Synthetic peptide corresponding to a region derived from internal residues of human lysine (K)-specific demethylase 5B
  • Target Name KDM5B
  • Alternative Names CT31; PLU1; PUT1; PLU-1; JARID1B; RBBP2H1A
  • Accession No. Swiss-Prot#: Q9UGL1
    NCBI Gene ID: 10765
    Gene Accssion: NP_006609
  • SDS-PAGE MW 176kd
  • Concentration 2.7mg/ml
  • Formulation Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:200-1:1000
Immunohistochemistry: 1:50-1:200

Images
  • KDM5B Antibody - SAB | Signalway Antibody

    Gel: 6%SDS-PAGE
    Lysates (from left to right): Hela and MCF-7 cell
    Amount of lysate: 40ug per lane
    Primary antibody: 1/200 dilution
    Secondary antibody dilution: 1/8000
    Exposure time: 10 minutes

  • KDM5B Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded Human brain tissue using #37681 at dilution 1/40.

Background
PLU-1 is a large (1,544 amino acid) nuclear protein that interacts with brain factor-1 (BF-1) and paired box 9 (PAX9), both of which are developmental transcription factors. PLU-1 belongs to the testis-cancer antigen group of proteins and is a member of the ARID family of DNA binding proteins. It is a multi-domain protein with strong transcriptional repression properties. PLU-1 shows restricted expression in adult tissues, with high expression in testis and transient expression in the pregnant mammary gland. Both the PLU-1 gene and the PLU-1 protein product are specifically upregulated in breast cancer. PLU-1 may be important in meiotic transcription because of its apparent association with chromatin.
    Please let us know if you have published research using #37681 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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