• Product NameFumarase Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityFumarase rabbit polyclonal antibody detects endogenous fumarase proteins.
• Target NameFumarase Antibody
• Alternative Names    FH; Fumarase; fumarate hydratase; Fumarate hydratase, mitochondrial; FUMH; HLRCC; LRCC; MCL; MCUL1
• Accession No. Swiss-Prot#:P07954
• UniprotP07954
• Gene ID2271;
• SDS-PAGE MW 50kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000~1:2000
  

Fumarase (FH) is an enzyme that catalyzes the reversible hydration/dehydration of fumarate to malate in the mitochondria. Fumarase deficiency is caused by a mutation in the fumarase gene in humans. Fumarase deficiency is one of the few known deficiencies of the Krebs cycle or tricarboxylic acid cycle, the main enzymatic pathway of cellular aerobic respiration.

• Western blot analysis of Hela (lane 1), mouse brain (lane 2) and HepG2 (lane 3), using #38002 diluted at 1:2,000.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting