• Product NameCK8 Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.8G8
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHu Ms Rt
• SpecificityThe CK8 Mouse Monoclonal antibody detects endogenous CK8 proteins.
• Target NameCK8
• Alternative Names    CARD2; CK-8; CK8; CYK8; cytokeratin 8; Cytokeratin-8; K2C8; K8; keratin 8
• Accession No. Swiss-Prot#:P05787
• UniprotP05787
• Gene ID3856;
• SDS-PAGE MW 54kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:2000~1:5000
  IHC dilution: 1:200
  

Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas.

• Western blot analysis of Hela (lane 1) and Mouse Brain (lane 2), using #38010 diluted at 1:4,000.
• IHC staining of human colon cancer tissue with CK8 mouse mAb(8G8) diluted at 1:200.
• Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1,CK8 Monoclonal Antibody(8G8) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1,CK8 Monoclonal Antibody(8G8) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,CK8 Monoclonal Antibody(8G8) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting