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Enzymes

MLC2 (Phospho-Ser15) Antibody #11590

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Catalog No. Package Size Price Quantity
50ul $219.00
100ul $319.00
Availability:Yes
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Description
  • Product NameMLC2 (Phospho-Ser15) Antibody
  • Host SpeciesRabbit
  • ClonalityPolyclonal
  • PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • ApplicationsWB IF
  • Species ReactivityHu
  • SpecificityThe antibody detects endogenous level of MLC2 only when phosphorylated at serine 15.
  • Immunogen TypePeptide-KLH
  • Immunogen DescriptionPeptide sequence around phosphorylation site of serine 15 (A-N-S(p)-N-V) derived from Human MLC2.
  • Target NameMLC2
  • ModificationPhospho
  • Alternative Names    MLC2; MRLC1; MYRL2
  • Accession No. Swiss-Prot#: P10916
    NCBI Gene ID: 4633
    NCBI Protein#: NP_006088.2
  • UniprotP10916
  • Gene ID4633;
  • SDS-PAGE MW 20kd
  • Concentration 1.0 mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C
Application Details
Background

Published Papers

PKM2 phosphorylates MLC2 and regulates cytokinesis of tumour cells.In Nat Commun on 2014 Nov 21 by Yuhui Jiang , Yugang Wang et al..PMID: 25412762, , (2014),
PMID: 25412762
PKM2 phosphorylates MLC2 and regulates cytokinesis of tumour cells.In Nat Commun on 2014 Nov 21 by Yuhui Jiang , Yugang Wang et al..PMID: 25412762, , (2014),
PMID: 25412762
Please let us know if you have published research using #11590 so that we can cite your reference.

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Application

WBWestern Blotting

IHCImmunohistochemistry

IFImmunofluorescence

ICCImmunocytochemistry

FCFlow Cytometry

IPImmunoprecipitation

EELISA

DBDot Blotting

ChIPChromatin Immunoprecipitation

GICAGold Immunochromatography Assay

NCNegative Control

Species Reactivity

HuHuman

MsMouse 

RtRat 

Dm Drosophila melanogaster

C Caenorhabditis elegans

MkMonkey

RbRabbit

B Bovine 

D Dog

PPig

HmHamster

ChHm Chinese Hamster 

ChkChicken  

ShpSheep