• Product NameRhoA antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total RhoA protein.
• Immunogen TypePeptide
• Immunogen DescriptionA synthetic peptide of human RhoA (NP_001655.1).
• Target NameRhoA
• Alternative Names    ARH12;ARHA;RHO12;RHOH12;Rho;RHOA;RhoA
• Accession No. Uniprot:P61586
  GeneID:387
• UniprotP61586
• Gene ID387
• SDS-PAGE MW 21KDa
• Concentration 1.0mg/ml
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified.

• Western blot analysis of extracts of various cell lines, using RhoA antibody.
• Immunohistochemistry of paraffin-embedded human placenta using RhoA Rabbit pAb.
• Immunofluorescence analysis of NIH-3T3 cells using RhoA Rabbit pAb.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting