• Product NamePPP2CB antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total PPP2CB protein.
• Immunogen TypePeptide
• Immunogen DescriptionA synthetic peptide of human PPP2CB.
• Target NamePPP2CB
• Alternative Names    PP2CB; PP2Abeta;
• Accession No. Swiss-Prot#: P62714
  NCBI Gene ID: 5516
• UniprotP62714
• Gene ID5516;
• SDS-PAGE MW 36kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:100 - 1:200
  IF 1:50 - 1:200

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit.

• Immunohistochemistry of paraffin-embedded rat kidney using PPP2CB at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human colon carcinoma using PPP2CB at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse kidney using PPP2CB at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of U-2 OS cells using PPP2CB at dilution of 1:100. Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using PPP2CB at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting