• Product NameRPS6KA3 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total RPS6KA3 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human RPS6KA3.
• Target NameRPS6KA3
• Alternative Names    CLS; RSK; HU-3; RSK2; MRX19; ISPK-1; p90-RSK2; pp90RSK2; MAPKAPK1B; S6K-alpha3;
• Accession No. Swiss-Prot#: P51812
  NCBI Gene ID: 6197
• UniprotP51812
• Gene ID6197;
• SDS-PAGE MW 84kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:20 - 1:50

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS).

• Immunohistochemistry of paraffin-embedded mouse stomach using RPS6KA3 at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of HeLa cells using RPS6KA3 . Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of mouse lung, using RPS6KA3 at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded rat kidney using RPS6KA3 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human stomach using RPS6KA3 at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting