• Product NameMLKL antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse
• SpecificityThe antibody detects endogenous level of total MLKL protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human MLKL.
• Target NameMLKL
• Alternative Names    9130019I15Rik;
• Accession No. Swiss-Prot#: Q8NB16
  NCBI Gene ID: 74568
• UniprotQ8NB16
• Gene ID197259;
• SDS-PAGE MW 54kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.

• Western blot analysis of extracts of various cell lines, using MLKL at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting