• Product NameLuciferase Polyclonal Antibody Cy3 Conjugated
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationPurified by Protein A.
• Applications ICC IF
• Species ReactivityFirefly
• Crossing ReactivityPhotinus pyralis (Common eastern firefly)
• Immunogen DescriptionKLH conjugated synthetic peptide derived from Firefly Luciferase
• ConjugatesCy3
• Excitation Emission512,550nm 570,615nm
• Target NameLuciferase
• Alternative Names    Luciferin 4-monooxygenase
• Accession No. NCBI Gene ID:7100
• UniprotO60602
• Gene ID7100;
• Concentration 1mg ml
• Formulation 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• Storage Shipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

• ICC=1:50-200 IF=1:50-200

Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting