• Product NameTUFM antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total TUFM protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human TUFM.
• Target NameTUFM
• Alternative Names    P43; EFTU; COXPD4; EF-TuMT;
• Accession No. Swiss-Prot#: P49411
  NCBI Gene ID: 7284
• UniprotP49411
• Gene ID7284;
• SDS-PAGE MW 49kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:10 - 1:100
  IP 1:50 - 1:200

This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17.

• Immunofluorescence analysis of MCF7 cells using TUFM . Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using TUFM at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded human esophageal cancer using TUFM at dilution of 1:100 (40x lens).
• Confocal immunofluorescence analysis of U2OS cells using TUFM Polyclonal at dilution of 1:100. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting