• Product NamePOLG2 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total POLG2 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human POLG2.
• Target NamePOLG2
• Alternative Names    HP55; POLB; PEOA4; POLGB; MTPOLB; POLG-BETA;
• Accession No. Swiss-Prot#: Q9UHN1
  NCBI Gene ID: 11232
• UniprotQ9UHN1
• Gene ID11232;
• SDS-PAGE MW 54kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:10 - 1:100

This gene encodes the processivity subunit of the mitochondrial DNA polymerase gamma. The encoded protein forms a heterotrimer containing one catalytic subunit and two processivity subunits. This protein enhances DNA binding and promotes processive DNA synthesis. Mutations in this gene result in autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions.

• Western blot analysis of extracts of various cell lines, using POLG2 at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded mouse testis using POLG2 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse kidney using POLG2 at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of MCF7 cells using POLG2 . Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded rat heart using POLG2 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human stomach using POLG2 at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting