• Product NamePOU2AF1 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total POU2AF1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fusion protein of human POU2AF1 (NP_006226.2).
• Target NamePOU2AF1
• Alternative Names    POU2AF1;BOB1;OBF-1;OBF1;OCAB
• Accession No. Uniprot:Q16633
  GeneID:5450
• UniprotQ16633
• Gene ID5450
• SDS-PAGE MW 27kDa
• Concentration 1.0mg/ml
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000

Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2. It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator (By similarity.

• Western blot analysis of extracts of various cell lines, using POU2AF1 antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting