• Product NameSKP2 (Ab-64) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total SKP2 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.62~66 (P-E-S-P-P) derived from Human SKP2.
• Target NameSKP2
• Alternative Names    FBXL1
• Accession No. Swiss-Prot#: Q13309
  NCBI Protein#: NP_005974.2.
• UniprotQ13309
• Gene ID6502;
• Target Species Human
• SDS-PAGE MW 48kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C

•  Western blotting: 1:500~1:1000

 Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration.

• Western blot analysis of extracts from 293T and JK cells using SKP2 (Ab-64) Antibody #21700.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting