• Product NameHis Tag Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• PurificationProtein A
• ApplicationsWB;IF;IP
• SpecificityThe antibody can detect His-Tag fusion proteins.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionRabbit polyclonal His-tag antibody was raised against a 6 amino acid peptide. The peptide sequence: HHHHHH
• Target NameHis
• Concentration 1.0mg/ml
• Formulation PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
• Storage This product is stable for several weeks at 4˚C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20˚C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

• WB 1:2000-1:10000;
  IF 1:200-1:1000;
  IP 1:50-1:200

 A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

• Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with His Tag antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Flag-His-MBP Lane 2: Myc-His-MBP Lane 3: HA-His-MBP Predicted band size: 50kDa Observed band size: 55kDa
• Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with His Tag antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Flag-His-MBP Lane 2: Myc-His-MBP Lane 3: HA-His-MBP Predicted band size: 50kDa Observed band size: 55kDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting