• Product NameTau(Phospho-Ser673) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB,IHC,IF,ELISA
• Species ReactivityHu Ms Rt
• SpecificityPhospho-Tau (S673) Polyclonal Antibody detects endogenous levels of Tau protein only when phosphorylated at S673.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human Tau around the phosphorylation site of Ser673.
• Target NameTau
• ModificationPhospho
• Alternative Names    MAPT; MTAPT; MTBT1; Neurofibrillary tangle protein; PHF-tau
• Accession No. Swiss-Prot: P10636
  NCBI Protein: NP_001116538.1
• UniprotP10636
• Gene ID4137;
• Calculated MW 50-85kD
• Concentration 1.0mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008],

• Western Blot analysis of NIH-3T3 cells using Phospho-Tau (S673) Antibody diluted at 1:500
• Western blot analysis of lysates from 293 cells treated with serum 10% 15', using Tau (Phospho-Ser673) Antibody. The lane on the right is blocked with the phospho peptide.
• Immunohistochemistry analysis of paraffin-embedded human brain, using Tau (Phospho-Ser673) Antibody. The picture on the right is blocked with the phospho peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting