• Product NameRNF214 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total RNF214 protein.
• Immunogen TypeProtein
• Immunogen DescriptionFusion protein corresponding to residues near the C terminal of human ring finger protein 214
• Target NameRNF214
• Accession No. Swiss-Prot:Q8ND24
  Gene Accssion:BC064581
• UniprotQ8ND24
• Gene ID257160;
• SDS-PAGE MW 78KD
• Concentration 1.2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry:1:10-1:50

RNF214 (RING finger protein 214) is a 703 amino acid protein that contains one RING-type zinc finger and is encoded by a gene that maps to human chromosome 11q23.3. Chromosome 11 houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are associated with defects in genes that maps to chromosome 11.

• Gel: 6%SDS-PAGE
  Lysates (from left to right): 823, PC3, 231 and hepg2 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/250 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 20 seconds
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #40325 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting