protein_function: Induces susceptibility to atherosclerosis (Bysimilarity). Cell adhesion molecule which is required forleukocyte transendothelial migration (TEM) under most inflammatoryconditions. Tyr-690 plays a critical role in TEM and is requiredfor efficient trafficking of PECAM1 to and from the lateral borderrecycling compartment (LBRC) and is also essential for the LBRCmembrane to be targeted around migrating leukocytes. Preventsphagocyte ingestion of closely apposed viable cells bytransmitting "detachment" signals, and changes function onapoptosis, promoting tethering of dying cells to phagocytes (theencounter of a viable cell with a phagocyte via the homophilicinteraction of PECAM1 on both cell surfaces leads to the viablecell"s active repulsion from the phagocyte. During apoptosis, theinside-out signaling of PECAM1 is somehow disabled so that theapoptotic cell does not actively reject the phagocyte anymore. Thelack of this repulsion signal together with the interaction of theeat-me signals and their respective receptors causes theattachment of the apoptotic cell to the phagocyte, thus triggeringthe process of engulfment). Isoform Delta15 is unable to protectagainst apoptosis. Modulates BDKRB2 activation. Regulatesbradykinin- and hyperosmotic shock-induced ERK1,2 activation inhuman umbilical cord vein cells (HUVEC)..
Human platelet,endothelial cell adhesion molecule-1(PECAM1), an important member of the immunoglobulin gene superfamily, is widely distributed on cells of the vascular system and mediates cellular interactions through both homophilic and heterophilic adhesive mechanisms. The function of PECAM1 in vitro has begun to be understood, but its function in vivo is yet to be established.1 The PECAM1 locus is on the long arm of chromosome 17, in the region q23-qter. To confirm this observation and obtain a more precise localization of the PECAM1 locus, fluorescence in situ hybridization was conducted. Together our data allowed assignment of the PECAM1 locus to the region 17q23.2 To examine the functional role of PECAM-1 in regulating platelet-collagen interactions, 2 different approaches were applied using recombinant human PECAM-1-immunoglobulin chimeras and platelets derived from PECAM-1-deficient mice.3 PECAM-1, an integral membrane protein with an essential role in TEM, is found in this compartment and constitutively recycles evenly along endothelial cell borders.4 The standard used in this kit is the product of gene recombinant expression(extracellular part), consisting of 574 amino acids(Q28-K601) with the molecular weight of 64.5KDa. After glycosylating, its molecular weight changes to 90-100KDa.