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Human APOA1 ELISA Kit

EK5684
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EK5684 96T $559.00

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Description
  • Product Name Human APOA1 ELISA Kit
  • Specificity Human
  • Crossing Reactivity There is no detectable cross-reactivity with other relevant proteins.
  • Immunogen Type E.coli,D25-Q267
  • Alternative Names Apolipoprotein A-I; Apo-AI; ApoA-I; Apolipoprotein A1; Proapolipoprotein A-I; ProapoA-I; Truncated apolipoprotein A-I; Apolipoprotein A-I(1-242); APOA1;
  • Accession No. P02647
  • Cell Localization Secreted.
Application Details

sensitivity:50pg ml
Detect Range:3.12ng ml-200ng ml
sample_type:cell culture supernates serum plasma(heparin EDTA) and urine.
capture_antibody:
detection_antibody:
gene_name:APOA1
protein_name:Apolipoprotein A-I
gene_full_name:Apolipoprotein A-I
tissue_specificity: Major protein of plasma HDL also found inchylomicrons. Synthesized in the liver and small intestine. Theoxidized form at Met-110 and Met-136 is increased in individualswith increased risk for coronary artery disease such as incarrier of the eNOSa b genotype and exposure to cigarette smoking.It is also present in increased levels in aortic lesions relativeto native ApoA-I and increased levels are seen with increasingseverity of disease..
sequence_similarities:
tmb_incubation:25-30min
research_category:cardiovascular|lipids / lipoproteins|lipid metabolism|cholesterol metabolism|signal transduction|kits/ lysates/ other|kits|elisa kits|cancer proteins elisa kits|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|lipoprotein elisa kits

Product Description
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human APOA1
Background
protein_function: Participates in the reverse transport of cholesterolfrom tissues to the liver for excretion by promoting cholesterolefflux from tissues and by acting as a cofactor for the lecithincholesterol acyltransferase (LCAT). As part of the SPAP complex,activates spermatozoa motility..
Apolipoprotein A1 is a protein that in humans is encoded by the APOA1 gene. This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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