• Product NameRecombinant Human Tumor necrosis factor receptor type 1-associated DEATH domain protein(TRADD)
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• PurificationGreater than 90% as determined by SDS-PAGE.
• Immunogen DescriptionExpression Region:1-312aa
  Sequence Info:Full Length
• Alternative Names    TNFRSF1A-associated via death domain
• Accession No. Q15628
• UniprotQ15628
• Gene ID8717;
• Calculated MW 61.2 kDa
• Tag Info N-terminal GST-tagged
• Target Sequence MAAGQNGHEEWVGSAYLFVESSLDKVVLSDAYAHPQQKVAVYRALQAALAESGGSPDVLQMLKIHRSDPQLIVQLRFCGRQPCGRFLRAYREGALRAALQRSLAAALAQHSVPLQLELRAGAERLDALLADEERCLSCILAQQPDRLRDEELAELEDALRNLKCGSGARGGDGEVASAPLQPPVPSLSEVKPPPPPPPAQTFLFQGQPVVNRPLSLKDQQTFARSVGLKWRKVGRSLQRGCRALRDPALDSLAYEYEREGLYEQAFQLLRRFVQAEGRRATLQRLVEALEENELTSLAEDLLGLTDPNGGLA
• Formulation Tris-based buffer50% glycerol
• Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C.
  Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.

The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF,ARF of CDKN2A by TRIP12: acts by interacting with TRIP12, leading to disrupt interaction between TRIP12 and isoform p19ARF,ARF of CDKN2A. Adapter molecule for TNFRSF1A,TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A,TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.

"The TNF receptor 1-associated protein TRADD signals cell death and NF-kappa B activation." Hsu H., Xiong J., Goeddel D.V. Cell 81:495-504(1995)
Research Topic:Others

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting