• Product NameSmoothened Agonist (SAG)
• Brief DescriptionInhibitors
• Purification99.19%
• Biological ActivitySAG is a potent Smoothened (Smo) receptor agonist (Kd = 59 nM); potently activates the Hedgehog signaling pathway in Shh-light 2 cells (EC50 ~ 3 nM).
• Target NameHedgehog/Smoothened antagonist
• CAS No. 912545-86-9
• Calculated MW 490.06
• Formulation C28H28ClN3OS
• Storage 3 years -20˚C powder;2 years -80˚C in solvent;

• Research Area:Other
  SMILES:CNC1CCC(CC1)N(CC1=CC=CC(=C1)C1=CC=NC=C1)C(=O)C1=C(Cl)C2=CC=CC=C2S1
  Pathways:GPCR/G Protein; Stem Cells
  Receptor:Smoothened
  Boling pt:
  Melting pt:
  Solubility:Ethanol: 40 mg/mL warmed (75.97 mM); Water: 100 mg/mL (189.92 mM); DMSO: 71 mg/mL warmed (134.84 mM)
  Appearance:White Powder
  Remark:For obtaining a higher solubility , please warm the tube at 37 ˚C and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20 ˚C for several month.

1. Chen JK, et al. Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14071-6.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting