• Product NameNEK4 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 241 and 474 of NEK4
• Target NameNEK4
• Accession No. Swiss-Prot:P51957
  Gene ID:6787
• UniprotP51957
• Gene ID6787;
• Concentration 0.4mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 95kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:100-1:250
  

Seems to act exclusively upon threonine residues.

• Sample (30 ug of whole cell lysate)
  A: A431
  B: H1299
  7.5% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using NEK4 antibody at 1: 100 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting