• Product NameUBE2F Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsIHC
• Species ReactivityHu, Ms, Rt
• SpecificityThe antibody detects endogenous levels of total UBE2F protein.
• Immunogen TypePeptide
• Immunogen DescriptionFusion protein of human UBE2F
• Target NameUBE2F
• Alternative Names    NCE2
• Accession No. Swiss-Prot#:Q969M7
  NCBI Gene ID:140739
  Gene Accssion:BC010549
• UniprotQ969M7
• Gene ID140739;
• Concentration 1.7
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• IHC dilution:1: 100-200
  

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

• The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 47452(UBE2F Antibody) at dilution 1/100.(Original magnification: 200)
• The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 47452(UBE2F Antibody) at dilution 1/100.(Original magnification: 200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting