• Product NameZWILCH Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsWB
• Species ReactivityHu, Ms
• SpecificityThe antibody detects endogenous levels of total ZWILCH ? protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide of human ZWILCH ?
• Target NameZWILCH
• Alternative Names    KNTC1AP; hZwilch
• Accession No. Swiss-Prot#:Q9H900
  NCBI Gene ID:55055
  Gene Accssion:NP_060445
• UniprotQ9H900
• Gene ID55055;
• Calculated MW 67 kDa
• Concentration 0.6
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• WB dilution:1:1000-5000
  

This protein is essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. And it is required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. ZWILCH gene is deleted in a patient suffering from colorectal cancer with chromosomal instability.

• Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: HT29 cells, hela cells, Primary antibody:47464(ZWILCH ? Antibody) at dilution 1/1750, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting