• Product NameMDFI Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsWB, IHC
• Species ReactivityHu, Rt
• SpecificityThe antibody detects endogenous levels of total MDFI protein.
• Immunogen TypePeptide
• Immunogen DescriptionFusion protein of human MDFI
• Target NameMDFI
• Alternative Names    I-MF; I-mfa
• Accession No. Swiss-Prot#:Q99750
  NCBI Gene ID:4188
  Gene Accssion:BC007836
• UniprotQ99750
• Gene ID4188;
• Calculated MW 25 kDa
• Concentration 1
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• WB dilution:1:500-2000
  IHC dilution:1: 50-300
  

This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures.

• Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane: Human placenta tissue lysate, Primary antibody:47720(MDFI Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
• The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 47720(MDFI Antibody) at dilution 1/55.(Original magnification: 200)
• The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 47720(MDFI Antibody) at dilution 1/55.(Original magnification: 200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting