• Product NameHADH Antibody
• PurificationImmunogen affinity purified.
• ApplicationsWB;IHC;ICC/IF
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionSynthetic peptide corresponding to Human HADH
• Alternative Names    3 ketoacyl Coenzyme A (CoA) thiolase alpha subunit antibody
  3 oxoacyl CoA thiolase antibody
  78 kDa gastrin binding protein antibody
  78 kDa gastrin-binding protein antibody
  ECHA antibody
  ECHA_HUMAN antibody
  GBP antibody
  HADH antibody
  HADHA antibody
  Hydroxyacyl Coenzyme A dehydrogenase/3 ketoacyl Coenzyme A thiolase/enoyl Coenzyme A hydratase (trifunctional protein) alpha subunit antibody
  LCEH antibody
  LCHAD antibody
  Long chain 3-hydroxyacyl-CoA dehydrogenase antibody
  Mitochondrial long chain 2 enoyl Coenzyme A (CoA) hydratase alpha subunit antibody
  Mitochondrial long chain L 3 hydroxyacyl Coenzyme A dehydrogenase alpha subunit antibody
  Mitochondrial trifunctional enzyme alpha subunit antibody
  Mitochondrial trifunctional protein alpha subunit antibody
  MTPA antibody
  Thiolase/enoyl Coenzyme A hydratase (trifunctional protein) alpha subunit antibody
  TP ALPHA antibody
  TP-alpha antibody
  Trifunctional enzyme subunit alpha mitochondrial precursor antibody
  
• Accession No. Swiss-Prot#:Q16836
• UniprotQ16836
• Gene ID3033;
• Calculated MW 34 kDa
• Formulation 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB 1:5000;
  IHC 1:1000;
  ICC/IF 1:100

Hydroxyacyl-Coenzyme A dehydrogenase also known as HADH is an enzyme which in humans is encoded by the HADH gene. This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. A deficiency is associated with 3-hydroxyacyl-coenzyme A dehydrogenase deficiency.

• Western blot analysis of HADH on different lysates with HADH at 1/5,000 dilution.
  Lane 1: HepG2 cell lysate (20 ug/Lane)
  Lane 2: MCF7 cell lysate (20 ug/Lane)
  Lane 3: Mouse liver tissue lysate (40 ug/Lane)
  Lane 3: Mouse kidney tissue lysate (40 ug/Lane)
  Lane 3: Rat kidney tissue lysate (40 ug/Lane)
  Predicted band size: 34 kDa
  Observed band size: 34 kDa
• Application: IF-Tissue
  Species: Human
  Site: liver
  Sample: Paraffin-embedded section
  Antibody concentration: 1/100
• Application: IF-Tissue
  Species: Rat
  Site: kidney
  Sample: Paraffin-embedded section
  Antibody concentration: 1/100
• Immunohistochemical analysis of paraffin-embedded human liver tissue with HADH at 1/1,000 dilution.
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• Immunohistochemical analysis of paraffin-embedded mouse liver tissue with HADH at 1/1,000 dilution.
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• Immunohistochemical analysis of paraffin-embedded rat liver tissue with HADH at 1/1,000 dilution.
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• Immunocytochemistry analysis of HeLa cells labeling HADH at 1/100 dilution.
  Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with HADH at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting