• Product NameSir2/SIRT1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationProtein A purified
• ApplicationsWB, ICC, IHC, IP
• Species ReactivityHu
• Immunogen Descriptionpeptide
• Alternative Names    Conserved NAD+ dependent histone deacetylase of the Sirtuin family involved in regulation of lifespan; plays roles in silencing at HML, HMR, telomeres antibody
  MAR1 antibody
  NAD dependent histone deacetylase SIR2 antibody
  Regulatory protein SIR2 antibody
  Silent information regulator 2 antibody
  Sir2p antibody
  
• Accession No. Swiss-Prot#:Q96EB6
• UniprotQ96EB6
• Gene ID23411;
• Calculated MW 110kDa
• Formulation 1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000
  IHC: 1:100
  ICC: 1:100
  IP: 1:50
  

The S. cerevisiae silent information regulator 2 (Sir2) protein is an NAD+-dependent histone deacetylase (HDACs) that plays a critical role in transcriptional silencing, genome stability, and longevity (1-2). Part of the Sirtuin family (Sir2, Sir3, Sir3), yeast Sir2 is the founding member of Class III HDACs. A human homologue of Sir2, SIRT1, regulates the activity of the p53 tumor suppressor and inhibits apoptosis (3-4).

• Western blot analysis on Jurkat cell lysate using anti-Sir2.
• Immunohistochemical analysis of paraffin-embedded human colon carcinoma using anti-Sir-2.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting