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CD133 Antibody

48362
Review: comment rank 5 pdf
  Catalog No. Package Size Price
48362-1 50ul $260.00
48362-2 100ul $390.00

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Description
  • Product Name CD133 Antibody
  • Host Species Mouse
  • Clonality Monoclonal
  • Clone No. D2-B1
  • Purification Peptide affinity purified
  • Applications IHC,ICC
  • Species Reactivity Hu, Ms
  • Immunogen Description Peptide
  • Alternative Names AC133 antibody
    Antigen AC133 antibody
    CD133 antibody
    CORD12 antibody
    Hematopoietic stem cell antigen antibody
    hProminin antibody
    MCDR2 antibody
    MSTP061 antibody
    OTTHUMP00000217744 antibody
    OTTHUMP00000217745 antibody
    OTTHUMP00000217746 antibody
    PROM1 antibody
    PROM1_HUMAN antibody
    Prominin I antibody
    Prominin like 1 antibody
    Prominin like protein 1 precursor antibody
    Prominin mouse like 1 antibody
    Prominin-1 antibody
    Prominin-like protein 1 antibody
    Prominin1 antibody
    PROML1 antibody
    RP41 antibody
    STGD4 antibody
  • Accession No. Swiss-Prot#:O43490
  • Calculated MW Predicted band size 97 kDa
  • Formulation 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage Store at -20˚C
Application Details


IHC: 1:50-1:200
ICC: 1:100-1:500

Images
  • CD133 Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD133 antibody. Counter stained with hematoxylin.

  • CD133 Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD133 antibody. Counter stained with hematoxylin.

  • CD133 Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD133 antibody. Counter stained with hematoxylin.

  • CD133 Antibody - SAB | Signalway Antibody

    ICC staining CD133 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • CD133 Antibody - SAB | Signalway Antibody

    ICC staining CD133 (green) in N2A cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Background
CD133 antigen also known as prominin-1 is a glycoprotein that in humans is encoded by the PROM1 gene. It is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localize to cellular protrusions. While the precise function of CD133 remains unknown, it has been proposed to act as an organizer of cell membrane topology.
Regerences
1. Kim MY et al. Accumulation of low-dose BIX01294 promotes metastatic potential of U251 glioblastoma cells. Oncol Lett 13:1767-1774 (2017).
2. Xi G et al. Targeting CD133 improves chemotherapeutic efficacy of recurrent pediatric pilocytic astrocytoma following prolonged chemotherapy. Mol Cancer 16:21 (2017).
    Please let us know if you have published research using #48362 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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