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TNNI2 Antibody

48458
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  Catalog No. Package Size Price
48458-1 50ul $260.00
48458-2 100ul $390.00

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Description
  • Product Name TNNI2 Antibody
  • Host Species Mouse
  • Clonality Monoclonal
  • Clone No. A8-F12
  • Purification ProA affinity purified
  • Applications WB, IHC, FC
  • Species Reactivity Hu
  • Immunogen Description Recombinant protein
  • Alternative Names AMCD 2B antibody
    AMCD2B antibody
    DA 2B antibody
    DA2B antibody
    fast skeletal muscle antibody
    Fast twitch skeletal muscle troponin I antibody
    fast-twitch isoform antibody
    FSSV antibody
    fsTnI antibody
    TNNI 2 antibody
    Tnni2 antibody
    TNNI2_HUMAN antibody
    tro antibody
    Troponin I antibody
    Troponin I fast skeletal muscle antibody
    Troponin I fast twitch 2 antibody
    Troponin I fast twitch isoform antibody
    Troponin I fast twitch skeletal muscle isoform antibody
    Troponin I skeletal fast antibody
    Troponin I type 2 (skeletal fast) antibody
    Troponin I type 2 antibody
  • Accession No. Swiss-Prot#:P48788
  • Calculated MW 21 kDa
  • Formulation 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
  • Storage Store at -20˚C
Application Details

WB: 1:500-1:1,000 FC: 1:100-1:200
IHC: 1:100-1:200


FC: 1:100-1:200

Images
  • TNNI2 Antibody - SAB | Signalway Antibody

    Western blot analysis of TNNI2 on human TNNI2 recombinant protein using anti-TNNI2 antibody at 1/1,000 dilution.

  • TNNI2 Antibody - SAB | Signalway Antibody

    Western blot analysis of TNNI2 on HEK293 (1) and TNNI2-hIgGFc transfected HEK293 (2) cell lysate using anti-TNNI2 antibody at 1/1,000 dilution.

  • TNNI2 Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue using anti-TNNI2 antibody. Counter stained with hematoxylin.

  • TNNI2 Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human striated muscle tissue using anti-TNNI2 antibody. Counter stained with hematoxylin.

  • TNNI2 Antibody - SAB | Signalway Antibody

    Flow cytometric analysis of NIH/3T3 cells with TNNI2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).

Background
Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. Myosin is a hexamer of 2 heavy chains (MHC) and 4 light chains (MLC) that interacts with actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. Troponin facilitates the interaction between actin and myosin by binding to calcium. Troponin is made up of at least two subunits, which are skeletal muscle skeletal muscle and slow skeletal muscle. Structures of skeletal muscle troponin are composed of Troponin C (the sensor), Troponin I (the regulator), and Troponin T (the link to the the regulator), and Troponin T C is dumbbell-shaped and has a hydrophobic pocket that increases the contractile force of muscle fibers. Troponin C has 2 isoforms: fast and slow. Fast troponin C has two calci um binding sites while slow / cardiac troponin C has a single calcium binding site.
Regerences
1. Ottenheijm, CA. et al. Altered myofilament function depresses force generation in patients with nebulin-based nemaline myopathy (NEM2). J Struct Biol 170:334-343 (2010).
2. Alapat, D.V. et al. Fiber-types of sarcomeric proteins expressed in cultured myogenic cells are modulated by the dose of myogenin activity. Cell Signal 21:128-135 (2009).
    Please let us know if you have published research using #48458 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
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    • PPig
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