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CD133 Antibody

48566
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  Catalog No. Package Size Price
48566-1 50ul $260.00
48566-2 100ul $390.00

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Description
  • Product Name CD133 Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Peptide affinity purified
  • Applications WB, ICC, IHC, FC
  • Species Reactivity Hu
  • Immunogen Description recombinant protein
  • Alternative Names AC133 antibody
    Antigen AC133 antibody
    CD133 antibody
    CORD12 antibody
    Hematopoietic stem cell antigen antibody
    hProminin antibody
    MCDR2 antibody
    MSTP061 antibody
    OTTHUMP00000217744 antibody
    OTTHUMP00000217745 antibody
    OTTHUMP00000217746 antibody
    PROM1 antibody
    PROM1_HUMAN antibody
    Prominin I antibody
    Prominin like 1 antibody
    Prominin like protein 1 precursor antibody
    Prominin mouse like 1 antibody
    Prominin-1 antibody
    Prominin-like protein 1 antibody
    Prominin1 antibody
    PROML1 antibody
    RP41 antibody
    STGD4 antibody
  • Accession No. Swiss-Prot#:O43490
  • Calculated MW 100 kDa
  • Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage Store at -20˚C
Application Details

WB: 1:1,000
IHC: 1:200
ICC: 1:200

FC: 1:100-1:200

Images
  • CD133 Antibody - SAB | Signalway Antibody

    Western blot analysis of CD133 on different cell lysates using anti-CD133 antibody at 1/1000 dilution.

  • CD133 Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD133 antibody. Counter stained with hematoxylin.

  • CD133 Antibody - SAB | Signalway Antibody

    ICC staining CD133 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • CD133 Antibody - SAB | Signalway Antibody

    ICC staining CD133 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • CD133 Antibody - SAB | Signalway Antibody

    ICC staining CD133 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • CD133 Antibody - SAB | Signalway Antibody

    Flow cytometric analysis of SHG-44 cells with CD133 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

  • CD133 Antibody - SAB | Signalway Antibody

    Flow cytometric analysis of HUVEC cells with CD133 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

Background
CD133 is a glycoprotein also known in humans and rodents as Prominin 1 (PROM1). It is the founding member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localizes to cellular protrusions. CD133 is expressed in hematopoietic stem cells, endothelial progenitor cells, glioblastomas, neuronal and glial stem cells and some other cell types. CD133 is recognized as a stem cell marker for normal and cancerous tissues.
Regerences
1. Yin AH, Miraglia S, Zanjani ED,et al. CD133, a novel marker for human hematopoietic stem and progenitor cells. Blood,1997; 90:5002-5012 2. Wuchter C,Ratei R,Spahn G,et al. Impact of CD133 (CD133) and CD90 expression analysis for acute leukemia immunophenotyping. Haematologica, 2001; 86:154-1613. Kuci S,Schumn M,Schlegel PG,et al. Phenotypic and functional characterization of mobilized peripheral blood CD133+CD34+ hematopoietic stem cell. Blood,1999; 94(Suppl 1):559a.
    Please let us know if you have published research using #48566 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
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