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Histone H1.3(Phospho-T17)+Histone H1.4(Phospho-T17) Antibody

13339
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  Catalog No. Package Size Price
13339-1 50ul $260.00
13339-2 100ul $390.00

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Description
  • Product Name Histone H1.3(Phospho-T17)+Histone H1.4(Phospho-T17) Antibody
  • Host Species Rabbit
  • Clonality Monoclonal
  • Clone No. SR38-03
  • Purification ProA affinity purified
  • Applications WB, ICC/IF, IHC
  • Species Reactivity Hu, Ms, Rt
  • Immunogen Description Synthetic phospho-peptide corresponding to residues surrounding Thr17 of human H1.4.
  • Alternative Names Histone H1.3
    Histone H1c
    Histone H1s-2
    HIST1H1DH1F3
    Histone H1.4
    Histone H1b
    Histone H1s-4
    HIST1H1EH1F4
  • Accession No. Swiss-Prot#:P10412
  • Calculated MW 30 kDa
  • Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage Store at -20˚C
Application Details

WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Images
  • Histone H1.3(Phospho-T17)+Histone H1.4(Phospho-T17) Antibody - SAB | Signalway Antibody

    Western blot analysis of Phospho-Histone H1.3(T17)+Histone H1.4(T17) on CRC cell lysates using anti-Phospho-Histone H1.3(T17)+Histone H1.4(T17) antibody at 1/500 dilution.

    Positive control:
    Lane 1: Untreated CRC whole cell lysates

    Lane2: CRC cells treated with 1.5ug/ml Colcemid for 12 hours whole cell lysates


  • Histone H1.3(Phospho-T17)+Histone H1.4(Phospho-T17) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-Histone H1.3(T17)+Histone H1.4(T17) antibody. Counter stained with hematoxylin.

  • Histone H1.3(Phospho-T17)+Histone H1.4(Phospho-T17) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded mouse colon cancer tissue using anti-Phospho-Histone H1.3(T17)+Histone H1.4(T17) antibody. Counter stained with hematoxylin.

  • Histone H1.3(Phospho-T17)+Histone H1.4(Phospho-T17) Antibody - SAB | Signalway Antibody

    ICC staining Phospho-Histone H1.3(T17)+Histone H1.4(T17) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Histone H1.3(Phospho-T17)+Histone H1.4(Phospho-T17) Antibody - SAB | Signalway Antibody

    ICC staining Phospho-Histone H1.3(T17)+Histone H1.4(T17) in CRC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Background
Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Regerences
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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