• Product NameSodium Potassium ATPase Rabbit mAb
• Clone No.ST0533
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, FC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionSynthetic peptide within Human ATP1A1 aa 39-83 / 1023.
• Alternative Names    ATPase Na+/K+ transporting alpha antibody
  adenosinetriphosphatase antibody
  AT1A1_HUMAN antibody
  ATP1A1 antibody
  ATP1A4 antibody
  ATP1AL2 antibody
  ATP1B antibody
  ATP1B1 antibody
  ATPase Na+/K+ transporting alpha 1 polypeptide antibody
  ATPase Na+/K+ transporting alpha 4 polypeptide antibody
  ATPase Na+/K+ transporting beta 1 polypeptide antibody
  ATPase, Na+/K+ transporting, alpha polypeptide-like 2 antibody
  ATPase, Na+/K+ transporting, beta 1 polypeptide antibody
  Beta 1-subunit of Na(+),K(+)-ATPase antibody
  Na(+)/K(+) ATPase alpha-1 subunit antibody
  Na(+)/K(+) ATPase alpha-4 subunit antibody
  Na+, K+ ATPase alpha subunit antibody
  Na+/K+ ATPase 1 antibody
  Na+/K+ ATPase 4 antibody
  Na+/K+ ATPase, alpha-D polypeptide antibody
  Na, K-ATPase beta-1 polypeptide antibody
  Na, K-ATPase, alpha-A catalytic polypeptide antibody
  Na,K-ATPase catalytic subunit alpha-A protein antibody
  Na,K-ATPase subunit alpha-C antibody
  polypeptide-like 2 antibody
  Sodium pump 1 antibody
  sodium pump 4 antibody
  Sodium pump subunit alpha-1 antibody
  sodium pump subunit alpha-4 antibody
  sodium pump subunit beta-1 antibody
  sodium-potassium ATPase catalytic subunit alpha-1 antibody
  sodium-potassium ATPase catalytic subunit alpha-4 antibody
  sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody
  sodium-potassium ATPase, alpha 4 polypeptide antibody
  sodium-potassium-ATPase, alpha 1 polypeptide antibody
  Sodium/potassium transporting ATPase alpha 1 chain antibody
  Sodium/potassium transporting ATPase subunit beta 1 antibody
  sodium/potassium-dependent ATPase beta-1 subunit antibody
  sodium/potassium-transporting ATPase alpha-4 chain antibody
  sodium/potassium-transporting ATPase beta-1 chain antibody
  Sodium/potassium-transporting ATPase subunit alpha-1 antibody
  sodium/potassium-transporting ATPase subunit alpha-4 antibody
  
• Accession No. Swiss-Prot#:P05023
• UniprotP05023
• Gene ID476;
• Calculated MW 100 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-5,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

The ubiquitously expressed sodium/potassium-ATPase (Na⁺/K⁺-ATPase) exists as an oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na⁺ ions and two K⁺ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na⁺/K⁺-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, α, β and γ, comprise the Na⁺/K⁺-ATPase oligomer. The α subunit contains the binding sites for ATP and the cations; the glycosylated β subunit ensures correct folding and membrane insertion of the α subunits. The small γ subunit co-localizes with the α subunit in nephron segments, where it increases the affinity of Na⁺/K⁺-ATPase for ATP. The β subunit, but not the γ subunit, is essential for normal activity of Na⁺/K⁺-ATPase.

• Western blot analysis of Sodium Potassium ATPase on A549 cell lysates using anti-Sodium Potassium ATPase antibody at 1/1,000 dilution.
• Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.
• ICC staining Sodium Potassium ATPase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Sodium Potassium ATPase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Hela cells with Sodium Potassium ATPase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting